Fig. 3

CircCTNNA1 acted as a sponged for miR-363-3p. a The putative binding sites of miR-363-3p in circCTNNA1 were presented. Dual-luciferase reporter assay (b, c) and RNA immunoprecipitation assay (d, e) were used to verify the interaction between miR-363-3p and circCTNNA1. f The expression of miR-363-3p in CRC tumor tissues (Tumor) and adjacent normal tissues (Normal) was tested by qRT-PCR. g QRT-PCR was used to measure the miR-363-3p expression in NCM460 cells and both CRC cell lines (SW620 and SW480). h The correlation between miR-363-3p and circCTNNA1 was evaluated using Pearson coefficient correlation. i The transfection efficiency of circCTNNA1 overexpressing vector was confirmed by testing circCTNNA1 expression in SW620 and SW480 cells. j, k SW620 and SW480 cells were transfected with vector, circCTNNA1, si-con or si-circCTNNA1#2. The expression of miR-363-3p was measured by qRT-PCR. *p < 0.05, ***p < 0.001