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Table 1 MetAFLP quantitative characteristics of the regenerants derived via somatic embryogenesis using tissue culture conditions of the M1–M9 trials and ANOVA results, including Tukey’s HSD tests

From: Barley somatic embryogenesis-an attempt to modify variation induced in tissue culture

   MetAFLP characteristics Tissue culture conditions
TCIV SV DMV DNMV CuSO4 (µM) AgNO3 (µM) Length of induction step (days)
ANOVA F 32.676 14.612 12.177 7.274    
p 0.0001 0.0001 0.0001 0.0001    
Trials M1 7.75c 2.01bc 1.92c 1.63bc 0.1 0 21
M2 8.23c 2.78bcd 1.92c 1.34c 0.1 10 28
M3 7.94c 2.21d 1.92c 1.53c 0.1 60 35
M4 7.85c 2.11d 2.40b 1.34c 5 60 28
M5 7.66c 2.11d 2.40b 1.34c 5 0 35
M6 8.13c 2.59 cd 2.40b 1.63bc 5 10 21
M7 9.67b 3.83a 2.40b 1.53c 10 10 35
M8 10.67ab 3.47ab 2.41a 2.22ab 10 60 21
M9 10.77a 3.37abc 2.41a 2.31a 10 0 28
  1. The TCIV, SV, DMV, and DNMV reflect tissue culture-induced variation, sequence variation, demethylation, and de novo methylation, respectively. The a, b, and c indicate Tukey’s HSD grouping