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Table 1 MetAFLP quantitative characteristics of the regenerants derived via somatic embryogenesis using tissue culture conditions of the M1–M9 trials and ANOVA results, including Tukey’s HSD tests

From: Barley somatic embryogenesis-an attempt to modify variation induced in tissue culture

  

MetAFLP characteristics

Tissue culture conditions

TCIV

SV

DMV

DNMV

CuSO4 (µM)

AgNO3 (µM)

Length of induction step (days)

ANOVA

F

32.676

14.612

12.177

7.274

   

p

0.0001

0.0001

0.0001

0.0001

   

Trials

M1

7.75c

2.01bc

1.92c

1.63bc

0.1

0

21

M2

8.23c

2.78bcd

1.92c

1.34c

0.1

10

28

M3

7.94c

2.21d

1.92c

1.53c

0.1

60

35

M4

7.85c

2.11d

2.40b

1.34c

5

60

28

M5

7.66c

2.11d

2.40b

1.34c

5

0

35

M6

8.13c

2.59 cd

2.40b

1.63bc

5

10

21

M7

9.67b

3.83a

2.40b

1.53c

10

10

35

M8

10.67ab

3.47ab

2.41a

2.22ab

10

60

21

M9

10.77a

3.37abc

2.41a

2.31a

10

0

28

  1. The TCIV, SV, DMV, and DNMV reflect tissue culture-induced variation, sequence variation, demethylation, and de novo methylation, respectively. The a, b, and c indicate Tukey’s HSD grouping