Barley somatic embryogenesis-an attempt to modify variation induced in tissue culture

Table 2 The arrangement of ANOVA statistics and Tukey’s HSD tests for trials in the verification process

		MetAFLP characteristics				Tissue culture conditions
		TCIV	SV	DMV	DNMV	CuSO₄ (µM)	AgNO₃ (µM)	Length of induction step (days)
ANOVA	F	9.117	0.192	16.997	7.009
ANOVA	p	0.001	0.827	0.0001	0.004
Trial	M10	12.76^a	4.34^a	4.41^a	1.26^ab	0.1	0	21
	M12	11.01^b	4.22^a	3.65^c	0.72^b	10	60	21
	M13	13.66^a	4.10^a	3.98^b	2.26^a	2.95	15	28

M10 states for control, whereas M12-M13 for the verification trials. TCIV, SV, DMV, and DNMV, reflect tissue culture-induced variation, sequence variation, demethylation, and de novo methylation. The a, b, and c indicate Tukey’s HSD grouping. The presented data does not include outliers that were excluded based on the Grubs test

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