Table 1 Gradient elution program during the chromatographic analysis
Time | Mobile phase A% | Mobile phase B% |
|---|---|---|
0.0 | Â | Â |
0.0 | 98.0 | 2.0 |
0.1 | 98.0 | 2.0 |
1.5 | 98.0 | 2.0 |
2.0 | 80.0 | 20.0 |
3.0 | 60.0 | 40.0 |
7.4 | 40.0 | 60.0 |
7.5 | 2.0 | 98.0 |
8.0 | 2.0 | 98.0 |
9.0 | 98.0 | 2.0 |
11.0 | 98.0 | 2.0 |
Time | Mobile phase A% | Mobile phase B% |
|---|---|---|
0.0 | Â | Â |
0.0 | 98.0 | 2.0 |
0.1 | 98.0 | 2.0 |
1.5 | 98.0 | 2.0 |
2.0 | 80.0 | 20.0 |
3.0 | 60.0 | 40.0 |
7.4 | 40.0 | 60.0 |
7.5 | 2.0 | 98.0 |
8.0 | 2.0 | 98.0 |
9.0 | 98.0 | 2.0 |
11.0 | 98.0 | 2.0 |