Fig. 5

Silencing of FRZB reversed the effects of circCNIH4-mediated in gastric cancer cells. MKN-74 and HGC-27 cells were transfected with circCNIH4 or circCNIH4 + si-FRZB. a and b The mRNA and protein levels of FRZB were detected in MKN-74 and HGC-27 cells. c The protein level of β-catenin was measured by western blot in MKN-74 and HGC-27 cells. d and e Proliferation of MKN-74 and HGC-27 cells was determined by MTT assay. f The protein level of Ki67 was measured by western blot in MKN-74 and HGC-27 cells. g Apoptosis of MKN-74 and HGC-27 cells was detected by flow cytometry assay. h and i Migration and invasion of MKN-74 and HGC-27 cells were assessed by transwell assay. *p < 0.05