Fig. 3

Fluorescence intensity measurements of cortical microtubules in the transition and fast elongation zone of wild-type and than/+ roots. Maximum intensity projections of serial CLSM sections of transition zone and fast elongation zone were used. Increased fluorescence intensity in than/+ was observed. (a) Effect of 4 h treatment with 400 nM oryzalin. (b) Effect of oryzalin on roots transplanted from control medium to substrate with oryzalin 200 nM for 6 h. Error bars indicate standard error. The intensity difference was statistically significant compared to control group (oryzalin-treated Col) (t-test, p < 0.001, n = 10). In all the figures the n corresponds to the number of cells measured in each root tip. At least three root tips were used for fluorescence intensity measurements