Fig. 8

DCB treatment increases microtubule resistance to oryzalin. Effects of combined treatment with DCB and oryzalin on wild-type roots. a, b Effect of 6 h treatment with 200 nM oryzalin on meristematic zone cells without a or after b 400 nM DCB treatment. Disordered phragmoplasts and incomplete cell walls can be observed in both cases (a, b). c Oryzalin-affected transition/fast elongation zone cells exhibit disrupted and depolymerized cortical microtubules. d Roots of seedlings transplanted for 28 h in medium with 400 nM DCB exhibit short cells with unaffected cortical microtubules. e In roots treated for 6 h with 400 nM DCB and 200 nM oryzalin, after been transplanted for 22 h in medium containing 400 nM DCB, cortical microtubules appear unaffected by oryzalin in the transition and fast elongation zone (e compare to d). Scale bars: 20 μm