Fig. 2

Sev treatment repressed cell proliferation and induced cell apoptosis in CRC. A, B The impacts of Sev (0%, 1.7%, 3.4% and 5.1%) on cell viability and cell colony-forming ability were presented by MTT and cell colony formation assays, respectively. C The effects of Sev (0%, 1.7%, 3.4% and 5.1%) on cell cycle were determined by DNA content quantitation assay in HCT116 and SW620 cells. D The effects of Sev (0%, 1.7%, 3.4% and 5.1%) on cell apoptosis were determined by Annexin V-FITC and PI double staining assay in HCT116 and SW620 cells. E Caspase-3 activity was determined by caspase-3 activity assay in HCT116 and SW620 cells treated with Sev at various doses (0%, 1.7%, 3.4% and 5.1%). *p < 0.05 (ANOVA with Tukey’s test)