Fig. 3

The migration and invasion of HCT116 and SW620 cells were repressed by Sev. A The influences of Sev (0%, 1.7%, 3.4% and 5.1%) on the migration of HCT116 and SW620 cells were revealed by wound-healing assay. B Transwell invasion assay was employed to demonstrate the impacts of Sev (0%, 1.7%, 3.4% and 5.1%) on the invasion of HCT116 and SW620 cells. C The impacts of Sev (0%, 1.7%, 3.4% and 5.1%) on the protein expression of Bax, t-caspase 3, C-caspase 3, MMP2 and MMP9 were determined by western blot analysis in HCT116 and SW620 cells. D Hsa_circ_0000231 expression was determined by qRT-PCR in HCT116 and SW620 cells treated with 0%, 1.7%, 3.4% and 5.1% of Sev. *p < 0.05 (ANOVA with Tukey’s test)