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Fig. 4 | Journal of Biological Research-Thessaloniki

Fig. 4

From: Sevoflurane inhibits malignant progression of colorectal cancer via hsa_circ_0000231-mediated miR-622

Fig. 4

Hsa_circ_0000231 knockdown repressed CRC process. A The knockdown efficiency of hsa_circ_0000231#1, si-hsa_circ_0000231#2 and si-hsa_circ_0000231#3 was determined by qRT-PCR in HCT116 and SW620 cells. B, C The impacts of hsa_circ_0000231 silencing on the viability and colony-forming ability were revealed by MTT and cell colony formation assays, respectively. D The effect of hsa_circ_0000231 absence on cell cycle was demonstrated by DNA content quantitation assay. E The effect of hsa_circ_0000231 absence on cell apoptosis was demonstrated by Annexin V-FITC and PI double staining assay. F The influence of hsa_circ_0000231 silencing on caspase-3 activity was unveiled by caspase-3 activity assay. G, H Wound-healing and transwell invasion assays were performed to demonstrate the impacts of hsa_circ_0000231 downregulation on the migration and invasion of HCT116 and SW620 cells, respectively. I The impacts of hsa_circ_0000231 silencing on the protein expression of Bax, t-caspase 3, C-caspase 3, MMP2 and MMP9 were determined by western blot analysis in HCT116 and SW620 cells. *p < 0.05 (ANOVA with Tukey’s test)

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