Fig. 6

Hsa_circ_0000231 directly interacted with miR-622. A Circular RNA Interactome online database was performed to predict the putative relationship between hsa_circ_0000231 and miR-622. B, C Dual-luciferase reporter and RIP assays were carried out to prove that hsa_circ_0000231 directly bound to miR-622. D The impacts between Sev treatment and hsa_circ_0000231 overexpression on miR-622 expression were demonstrated by qRT-PCR in HCT116 and SW620 cells. E, G MiR-622 expression was detected by qRT-PCR in 47 pairs of CRC and paracancerous normal colorectal tissues as well as NCM460, HCT116 and SW620 cells. F Spearman correlation analysis was conducted to disclose the linear relationship between hsa_circ_0000231 and miR-622 expression in CRC tissues. H The influences of various concentrations of Sev (0%, 1.7%, 3.4% and 5.1%) on miR-622 expression were determined by qRT-PCR in HCT116 and SW620 cells. *p < 0.05 (two-tailed Student’s t-tests, Wilcoxon rank-sum test, ANOVA with Tukey’s test and Spearman’s correlation test)