Fig. 1

Architecture of the domains of E2A. The domain architecture of CBP/P300 is shown in the middle, illustrating the four activation domains (AD1, DES, AD3, and AD2) and the C-terminal bHLH domain. The yellow shaded box represents AD1, and the core PCET motif of AD1 is represented by the small blue rectangle in the yellow shaded box. The dotted box represents the DES domain, and the N2B motif is represented by the small gold rectangle in the middle of the dotted box. The green box represents AD3, the orange box represents AD2, and the red box represents the bHLH domain. The numbers indicate the positions of the residues at both ends of these domains, AD1 (residues 1–99), DES domain (residues 100–239), AD3 (residues 211–298), AD2 (residues 310–430) and bHLH domain (residues 547–608). The structures of each domain are also shown and labeled. Top panel: The E2A PCET motif in complex with the ETO NHR1 domain (PDB: 2KNH); the E2A N2B motif in complex with the ETO NHR2 domain (PDB: 4JOL); the structure of the complex formed between the E2A bHLH domain and the SCL bHLH domain and E-box (PDB: 2YPB). Bottom panel: The PCET motif in complex with the CBP KIX domain (PDB: 2KWF); the structure of the complex formed between E2A AD1 (residues 1–37) and the CBP TAZ2 domain (PDB: 2MH0). The structures of the complex formed between E2A AD2 and NHR1, NHR2 or TAZ2 and the complex composed of E2A AD3 and TFIID was unclear