Fig. 3

The interaction of AD1 with CBP or ETO. a Sharing mode of the interaction of AD1 with the domains of CBP or ETO. The residues Leu16, Leu19, and Leu20 of E2A are shown and labeled; the hydrophobic grooves on the surface of the domains of CBP or ETO are shown in gray and white. The 3D structure was obtained from the Protein Data Bank (PDB: 2MH0) and was modified using Discovery Studio 3.0 software. b Characteristics of the interaction of AD1 with NHR1/eTAFH. Top panel: The structure of eTAFH was obtained from the Protein Data Bank (PDB: 2KNH); the region marked in yellow on the domains represents the hydrophobic residues in the domains that bind to AD1; the polar residues on the domains that bind to AD1 are shown and labeled in red. Bottom panel: The sequences of HEB-PCET (12–27) were obtained from the Protein Data Bank (PDB: 2KNH); the hydrophobic residues in the sequences that contribute to the interaction with the NHR1/eTAFH domain of ETO are represented by yellow squares, and the polar residues involved in the interaction are marked in red. The red dotted line represents hydrogen bonds. c Top panel: The structure of KIX was obtained from the Protein Data Bank (PDB: 2KWF); the region marked in yellow on the domains represents the hydrophobic residues in the domains that bind to AD1; the polar residues in the domains that bind to AD1 are shown and labeled in red. Bottom panel: The sequences of HEB-PCET (12–27) are shown; the hydrophobic residues in the sequences that contribute to the interaction with the KIX domain of CBP are represented by yellow squares, and the polar residues involved in the interaction are marked in red. The red dotted line represents hydrogen bonds. d Top panel: The structure of the TAZ2 domain was obtained from the Protein Data Bank (PDB: 2MH0); the region marked in yellow on the domains represents the hydrophobic residues in the domains that bind to AD1; the polar residues of the domains that bind to AD1 are shown and labeled in red. Bottom panel: The sequences of E2A-PCET (11–29) were obtained from the Protein Data Bank (PDB: 2MH0); the hydrophobic residues in the sequences that contribute to the interaction with the KIX domain of CBP are represented by yellow squares, and the polar residues involved in the interaction are marked in red. The red dotted line represents hydrogen bonds