Fig. 5
Nesfatin-1 protected H9c2 cells against CoCl2-induced hypoxic injury by inhibiting MAPK and Notch1 signaling pathways. H9c2 cells were treated with 800 µM CoCl2 for 48 h before treatment with nesfatin-1 (20, 60 and 80 nM) for 24 h. The expression of MAPK pathway members (A) and Notch1 pathway members (B) was detected by Western blot. Data are presented as mean  ±  SD. *p  <  0.05 vs. control group (0 µM CoCl2); #p  <  0.05 vs. CoCl2 alone group (800 µM CoCl2 and 0 nM nesfatin-1)