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Fig. 2 | Journal of Biological Research-Thessaloniki

Fig. 2

From: PTD-mediated delivery of α-globin chain into Κ-562 erythroleukemia cells and α-thalassemic (HBH) patients’ RBCs ex vivo in the frame of Protein Replacement Therapy

Fig. 2

Western Blot of recombinant protein extracts, separated on 15% SDS-PAGE, transferred and immunoblotted with anti-His.IgG. A The bacterial-IBs’ protein extracts (in 1 M l-Arg, pH 8.0). Lane 1: protein molecular mass marker; Lane 2: 10xHis-XaSITE-TAT-α-globin-HA (~ 20.2 kDa); Lane 3: protein molecular mass marker; Lane 4: 10xHis-XaSITE-α-globin-HA (~ 18.7 kDa); B The soluble-purified by Ni2+-NTA chromatography-recombinant proteins, eluted with 10 mM imidazole. Lane 1: protein molecular mass marker; Lane 2: 10xHis-XaSITE-TAT-α-globin-HA (~ 20.2 kDa); Lane 3: 10xHis-XaSITE-α-globin-HA (~ 18.7 kDa). Higher bands at 29—41 kDa indicate the corresponding protein dimers, while lower bands may indicate proteolysis products

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