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Fig. 7 | Journal of Biological Research-Thessaloniki

Fig. 7

From: PTD-mediated delivery of α-globin chain into Κ-562 erythroleukemia cells and α-thalassemic (HBH) patients’ RBCs ex vivo in the frame of Protein Replacement Therapy

Fig. 7

Western Blot of electrophoresed cell lysates derived from K-562 cells, transduced with 50 μg ml−1 soluble 10xHis − XaSITE − TAT − α-globin − HA, immunoblotted using anti-HA.IgG. Lane 1: protein molecular mass marker; Lane 2: lysate from control (untreated) cells, 30 min; Lane 3: lysate from cells transduced with 10xHis − XaSITE − TAT − α-globin − HA, incubated for 30 min.; Lane 4: lysate from control (untreated) cells, 1 h; Lane 5: lysate from cells transduced with 10xHis − XaSITE − TAT − α-globin − HA, incubated for 1 h

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