Fig. 8
Western Blot of electrophoresed cell lysates derived from HbH patients’ peripheral RBCs (Patient 3), transduced with bacterial-IBs, enriched in 10xHis-XaSITE-TAT-α-globin-HA, immunoblotted using anti-His.IgG and anti-GAPDH.IgG. Patient 3: Lane 1: protein molecular mass marker; Lane 2: lysate from control (untreated) cells, 48 h; Lane 3: lysate from cells incubated with 1 M l-Arg, for 48 h; Lanes 4–6: lysate from cells transduced with bacterial-IBs, enriched in 10xHis-XaSITE-TAT-α-globin-HA, incubated for 48 h