Fig. 3

Prx6 regulates proliferation and tumor growth in HCT116. A Ten days after seeding HCT116 control, Prx6 overexpressing, and siPrx6 cells, they were stained with 5% crystal violet. The number of colonies from three independent experiments. B The proliferation rate of HCT116 Prx6 overexpressing cells or Prx6-knockdown cells were detected with IncuCyte until 60 h. C Photos of mice on day 21. Control, Prx6-overexpressed, siPrx6 HCT116 cells were intraperitoneally injected into nude mice. D Tumor volume was measured using a caliper at the appointed time. Data are expressed as mean ± SD (n = 3). *p < 0.05, **p < 0.01, and ***p < 0.001