Fig. 2

Effects of circ_0000620 knockdown on the proliferation, tube formation and invasion of GC cells. HGC27 and AGS cells were transfected with sh-NC or sh-circ_0000620 for 36 h. A The expression of circ_0000620 was detected by qRT-PCR. B Cell viability was analyzed by CCK-8 assay. C The ability of colony formation was tested through colony formation assay. D Wound healing assay was implemented to assess cell migration capacity. E The invasive ability was assessed by transwell invasion assay. F The angiogenic ability was estimated through tube formation assay. G, H The expression of metastasis-associated protein markers (MMP2, E-cad and N-cad) was analyzed by western blot assay. Three repetitions were performed in each experiment, with three parallels every time. *p < 0.05