Fig. 2
From: SNP-based genetic linkage map of tobacco (Nicotiana tabacum L.) using next-generation RAD sequencing
Linkage maps based on the reference genome. This was constructed with a total of 8664 SNPs (p < 0.01) which generated 4138 markers mapping 24 linkage groups (LGs) successfully with a total length of 1944.74 cM. The LGs distance ranged from 33.58 to 129.176 cM. Six LGs contained over 220 marker loci and for these LGs Haldane’s map unit is used while for other LGs we used Kosambi’s map unit. The LG09, LG23 and LG24 were the shortest LGs, spanning 73.937–107.485 cM, respectively, and comprising 65 loci, whereas LG05 was the longest LG, spanning 60.73 cM and containing 494 loci with a marker density of 0.123 cM/locus. The marker densities ranged from 0.117 cM/locus in LG12 to 1.679 cM/locus in LG23, resulting in an average distance of 0.712 cM between markers for the entire map