Figure 1
From: Cell culture, sex determination and single cell cloning of ovine transgenic satellite cells in vitro
Primary cultures and identification of PSCs derived from mechanical and enzymatic disaggregation. (A) Enzymes treatment yielded the highest number of cells compared with mechanical disaggregation. (B) Desmin, Pax7 and CD34 were amplified with primers designed to produce an 101, 106 and 858-bp product in the primary satellite cells, respectively. Marker is 600 bp DNA ladder.