Table 2 Primer sequences, PCR conditions and PCR product sizes of ZFX/ZFY, HMG and amelogenin (AMEL) loci
From: Cell culture, sex determination and single cell cloning of ovine transgenic satellite cells in vitro
Gene | Primer sequence | T (°C) a | Product size (bp) | |
|---|---|---|---|---|
Male | Female | |||
ZFX/Yb | ||||
Forward | 5΄-ATAATCACATGGAGAGCCACAAGCT-3΄ | 58 | 445 | 445 |
Reverse | 5΄-GCACTTCTTTGGTATCTGAGAAAGT-3΄ | 58 | ||
HMG box of SRY genec | ||||
Forward | 5΄-TGAACGCTTTCATTGTGTGGTC-3΄ | 58 | 162 | - |
Reverse | 5΄-GCCAGTAGTCTCTGTGCCTCCT-3΄ | 58 | ||
Beta-actinc | ||||
Forward | 5΄-CCTTCAACACCCCTGCCATG-3΄ | 58 | 298 | 298 |
Reverse | 5΄-TGCTCGAAGTCCAGGGCC-3΄ | 57 | ||
Amelogenin (AMEL)d | ||||
Forward | 5΄-TTCTCACCAGTACCCTTCCTA-3΄ | 55 | 395 | 458 |
Reverse | 5΄-TCAGAGGCAGGTCAGGAAGCA-3΄ | 55 | ||