Figure 13

Maximum likelihood (ML) tree of concatenated, nuclear-encoded 18S and 5.8S + ITS2 sequence of Paralia. The concatenated sequence consists of a segment of the 18S gene and a 5.8S + ITS 2 barcode region (Stephanopyxis palmeriana is the outgroup; taxa aggregate into three groups, clades I, II and III with bootstrap support (ML/MP/NJ). Separation valve type for each clade is denoted as per Figure 10. The number in parentheses, next to the taxon name represents the number of clones used in construction of the tree if not all are shown (i.e., P. guyana ‘smooth’ genodeme and P. marina). Representative clones were selected based on methods used to generate Figure 10 (see text for explanation).