Table 5 Standard methods used for microbial analyses
Determination | Methodology | Medium, temperature and incubation time |
|---|---|---|
Aerobic Mesophilic Count (AMC) | ISO 4833:2003 | Plate Count Agar, 30°C, 72 hrs |
Enterobacteriaceae | ISO 21528–2:2004 | Violet Red Bile Glucose Agar, 37°C, 24 hrs |
E. coli β-glucuronidase positive | ISO 16649–2:2010 | Tryptone Bile Glucuronide Agar, 44°C, 24 hrs |
Coagulase positive staphylococci | ISO 6888–1:1999 Amend. 1:2003 | Baird Parker Agar, 37°C, 24–48 hrs |
Sulphite-reducing anaerobic organisms | ISO 15213:2003 | Iron Sulphite Agar, 37°C, 24 hrs |
L. monocytogenes | ISO 11290–2:1998 | ALOA Agar, 37°C, 24–48 hrs |