Two single nucleotide polymorphisms sites in α1-AT gene and their association with somatic cell score in Chinese Holstein cows

Table 1 Primer sequence, product size, location and annealing temperature of α1-AT gene in Chinese Holstein cows

Primer	Primer sequence (5′ → 3′)	Product size (bp)	Location^a	Annealing temperature (°C)
P1	F: GCCATTGTTCTGAGTCTTTC	978	5216–5235	54
P1	R: TTCCCTAACCCTATTTGATT	978	6174–6193	54
P2	F: GGCACCAACTGGAAAGAACAAC	175	8060–8081	59
P2	R: AGCCCTATCGCTGAAGACCT	175	8215–8234	59
P3	F: CCTTTGCGATGCTCTCCCTG	149	5616–5635	55
P3	R: CTGGTGGTTTGGCTGATT	149	5747–5764	55

ISSN: 2241-5793