Fig. 6
From: Bone marker gene expression in calvarial bones: different bone microenvironments
TaqMan analysis of skeletogenesis markers: FGF2 and FGFR1 expression in calvariae of C57BL/KalwRiJHsD mice. RNA was extracted with cDNA synthesized from frontal (F), parietal (P) and interparietal (IP) bones. The relative expression of skeletogenesis marker genes was adjusted to the relative expression of a housekeeping gene, β-actin. There was a significant increase in the relative expression of FGF2 (a) in frontal and parietal bones compared to interparietal bone. In addition, there was no difference in the relative expression of FGF2 between frontal and parietal bones. On the other hand, the relative expression of FGFR1 (b) genes was significantly expressed in parietal bone compared to frontal and interparietal bones