Fig. 5
From: Immune cell counts and signaling in body fluids of cows vaccinated against Clostridium difficile
Total SCC in milk and the associated ratio of phagocytes to lymphocytes. Within 31 treatment weeks (TW), the recorded total SCC in milk are shown in groups [“Ctr” control animals (n = 5), “LR” treated, low responder animals (n = 4), “HR” treated, high responder animals (n = 5)] as LSM ± SD on the lower vertical axis. The gray dotted line at 200 thousand cells per milliliter marks the commonly used cut-off value for normal SCC. The upper line chart depicts the ratio of phagocytes to lymphocytes in milk. The routes of vaccination [nasal (N), percutaneous (PC), subcutaneous (SC) and intracutaneous (IC), incomplete Freund’s adjuvant (iFA)] are presented on the upper abscissa on the immunization (IM) dates. Two gray bars represent the IM times using the MucoCD-I vaccine batches A or B. The asterisks (p < 0.05) indicate the inner-group deviations in relation to the dates before the initial uses of both MucoCD-I vaccine batches. The significant differences (p < 0.05) between groups on the same dates are marked by lower case letters (a, b, c)