Table 3 Physicochemical properties of selected potential biomarkers using ProtParam, ProtScale, and PeptideMass tools
Potential biomarkers | Formula | Total amino Acids | Molecular weight | Theoretical pI | Ext. coefficient | Estimated half-life (h) | Instability index (II) | Hydropathicity (GRAVY) | Monoisotopic mass |
|---|---|---|---|---|---|---|---|---|---|
WIF1 | C1813H2808N518O524S40 | 379 | 415,27.80 | 7.84 | 46,920 | 30.0 | 54.70 | − 0.264 | 41,499.78 |
SPON1 | C3929H6190N1114O1225S73 | 807 | 90,973.40 | 5.85 | 149,205 | 30.0 | 61.34 | − 0.604 | 90,913.59 |
LYZ | C719H1146N220O207S11 | 148 | 16,537.00 | 9.38 | 36,940 | 30.0 | 27.71 | − 0.195 | 16,526.28 |
GPRC5B | C2072H3244N526O542S23 | 6407 | 44,933.13 | 8.06 | 65,930 | 30.0 | 42.45 | 0.52 | 44,903.60 |
PTPRE | C3622H5651N979O1040S34 | 700 | 80,641.69 | 6.57 | 97,010 | 30.0 | 35.24 | − 0.342 | 80,589.99 |
ZFP36L2 | C2219H3438N650O702S19 | 494 | 51,062.78 | 8.52 | 15,900 | 30.0 | 69.83 | − 0.387 | 51,030.80 |
HBB | C729H1128N196O202S4 | 147 | 15,998.41 | 6.74 | 15,595 | 30.0 | 6.16 | 0.014 | 15,988.29 |
HBB | C497H770N134O146S2 | 101 | 11,022.51 | 6.03 | 12,490 | 7.2 | 1.52 | − 0.239 | 11,015.64 |
PHF15 | C3798H6018N1078O1203S43 | 789 | 87,408.65 | 5.15 | 92,360 | 30.0 | 57.12 | − 0.639 | 87,353.08 |
LMCD1 | C1782H2822N502O539S29 | 365 | 40,832.79 | 8.27 | 53,860 | 30.0 | 47.12 | − 0.565 | 40,806.07 |
KRT35 | C2144H3464N620O715S31 | 455 | 50,360.67 | 4.85 | 37,245 | 30.0 | 61.99 | − 0.445 | 50,328.51 |
VAV3 | C3857H6055N1055O1148S35 | 753 | 86,695.99 | 6.41 | 88,920 | 30.0 | 42.18 | − 0.567 | 86,640.81 |