Fig. 6

Tan IIA declined Aβ-induced increase in COX-2 expression and PGE2 secretion, and activation of NF-κB pathway in SH-SY5Y cells. SH-SY5Y cells were treated with Tan IIA (5 or 10 μM) for 1 h, then exposed to Aβ (25 μM) for 48 h. a The mRNA level of COX-2 was detected by qRT-PCR analysis. b The protein level of COX-2 was measured by western blot analysis. c The concentration of PGE2 in culture supernatant was determined by ELISA assay. d qRT-PCR analysis examined the mPGES-1 mRNA level. e NF-κB levels was determined by ELISA assay. f The protein levels of MCL-1 and cyclin D1 was detected by western blot analysis. *p < 0.05, **p < 0.01 vs. respective control