Table 3 PCR primers used in the analyses of green-algae strains isolated from freshwaters of Greece
From: Polyphasic taxonomy of green algae strains isolated from Mediterranean freshwaters
Primer | Target gene-region | Sequence (5′–3′) | Size (bp) | References | Conditions |
|---|---|---|---|---|---|
EukA | 18S rRNA | AACCTGGTTGATCCTGCCAGT | 1750 | [78] | Initial denaturation step at 95 °C for 5 min, 35 cycles consisting of denaturation at 95 °C for 60 s, annealing at 55 °C for 60 s and elongation at 72 °C for 90 s; a final 7-min elongation step at 72 °C was included |
EukB | TGATCCTTCTGCAGGTTCACCTAC | ||||
U1391R | GGGCGGTGTGTACAARGR | [79] | |||
ITS-AF | ITS | CGTTTCCGTAGGTGAACCTGC | 700 | [80] | Initial denaturation step at 94 °C for 4 min, 35 cycles consisting of denaturation at 95 °C for 60 s, annealing at 58 °C for 2 min and elongation at 72 °C for 2 min; a final 7-min elongation step at 72 °C was included |
ITS-BR | CATATGCTTAAGTTCAGCGGG T | ||||
rbcL1-20 | rbcL | ATGGTTCCACAAACAGAAAC | 1100 | [81] | |
rbcL1181-1160 | AAGATTTCAACTAAAGCTGGCA |