Fig. 3
From: Modulation of RECK levels in Xenopus A6 cells: effects on MT1-MMP, MMP-2 and pERK levels
Transfection of full-length HA-tagged RECK constructs in A6 cells resulted in increased RECK levels. a Immunoblot analysis was used to confirm overexpression of RECK protein following transfection of RECK cDNA constructs. Mock-transfected and GFP-transfected A6 cells were used as controls. Protein was extracted from cells 24 h following transfection. β-actin is used as a loading control. Left panel demonstrates increased levels of RECK protein compared to control using a RECK antibody, while right panel confirms the presence of HA-tagged proteins only in RECK-transfected A6 cells using an HA antibody. b Graphed data is based on 3 biological replicates (mean ± SD) and normalized to control (set to 100%). Data is analyzed via t-test; ***, p ≤ 0.001