Fig. 1
From: TRAF3 can interact with GMEB1 and modulate its anti-apoptotic function
Identification of GMEB1 as a TRAF3-interacting protein by the yeast two hybrid system. Yeast colonies co-transformed with a plasmid expressing a fusion of GMEB1 to the Gal4 transactivating domain (pACTGMEB1) and a plasmid expressing a Gal4 DNA-binding domain fusion with TRAF3(12–568) (pAS1TRAF3) or LAMIN1 (pAS1LAMIN1) were tested for their ability to transactivate a Gal4-dependent β-galactosidase reporter gene using a filter lift assay