Fig. 3

CCND2 AS1 inhibits cell proliferation by arresting the cell cycle at the G1/S phase in cervical cancer cell lines. a, b The distribution of cell cycle phases analysis by flow cytometry between enforced expression of pCCND2 AS1 and control (pcDNA3.1) in HeLa and SiHa cell lines. The percentages of cells in the G1, S, and G2/M phases are shown in the bar chart as the mean ± SD of three independent experiments. *p < 0.05, **p < 0.01. c, d Flow cytometry analysis of the distribution of cell cycle phases in negative control (NC) and CCND2 AS1 SiRNA2 in HeLa and SiHa cell lines. The percentages of cells in the G1, S, and G2/M phases are shown in the bar chart as the mean ± SD of three independent experiments. *p < 0.05