Fig. 1
From: Application of antibody phage display to identify potential antigenic neural precursor cell proteins
Proteins contained in 20 μg of a NPCs lysate were separated in a 12% polyacrylamide gel and transferred onto PVDF membrane. Individual strips were probed with lane 1: immunized serum 2 and lane 2: the pre immune serum at a 1:1000 dilution in blocking buffer. A HRP-conjugated anti mouse IgG antibody (Cell Signaling, 7076) diluted 1:2000 in blocking buffer, served as secondary antibody. Blot was visualized with the ECL reagent on a autoradiography film. Arrows indicate the positions of the 58 and 80 kDa molecular mass standards