Application of antibody phage display to identify potential antigenic neural precursor cell proteins

Table 1 Titration results after each biopanning round

Panning round	Phage input (pfu)^a	Phage output (pfu)^b	% Bound^c	Enrichment factor^d
1	9.5 × 10¹¹	1.20 × 10⁶	1.20 × 10⁻⁶	–
2	7.3 × 10¹¹	0.80 × 10⁶	1.00 × 10⁻⁶	0.83
3	2.0 × 10¹¹	8.48 × 10⁷	4.24 × 10⁻⁴	424

^aNumber of phage pfu incubated with the antigens immobilized on the nitrocellulose strip
^bNumber of phage pfu eluted after the incubation above
^cPercentage of bound phage calculated as the ratio phage output/phage input × 100
^dRatio of the percentage of bound phage at round n vs the percentage of bound phage at round n–1

ISSN: 2241-5793