Fig. 2

Cell adhesion molecules in Pleomorphic Adenoma. a Dsg-2: Membranous or diffuse cytoplasmic staining at the intercellular contacts of neoplastic cells of all architecture types except luminal surfaces of luminal cells and basal surfaces of non-luminal cells. No positivity in stromal cells (Envision/HRP × 100). b Beta4-integrin: Strong membranous positivity at cell-basal membrane contacts, intercellular connections of the great majority of cells and stromal cells (Envision/HRP x200). c HCAM (CD44s): Strong membranous expression in the majority of neoplastic cells of all structures especially in stromal cells, cells of solid islands, non-luminal cells and intercellular contacts between luminal and non-luminal cells (Envision/HRP x200). d ICAM-1: Fibroblastoid stromal cells mainly in myxoid areas, linear positivity at the luminal surface of luminal ductal cells and moderate expression in some cells of solid islands (Envision/HRP x200). Strong immunoreactivity of basolateral portion of serous cell membrane and basal cells of interlobular ducts