Fig. 4

Circ_0000396 affects the growth and inflammation of RASFs by binding to miR-203. RASFs were transfected with pcDNA, circ_0000396, circ_0000396 + miR-NC, circ_0000396 + miR-203. After transfection, a the expression of miR-203 was measured by qRT-PCR; b, c RASF proliferation was analyzed by colony formation assay and CCK-8 assay, respectively; d the apoptosis of RASFs was measured by flow cytometry; e the expression of Ki67, cleaved caspase3 and cleaved PARP expression in RASFs was detected using western blot; f–i The level of inflammatory cytokines IL-6, IL-1β, IL-8 and TNF-α in RASFs was detected by ELISA assay. ^*p < 0.05, ^**p < 0.01, ^***p < 0.001, ^****p < 0.0001 (ANOVA)