Fig. 7

Circ_0000396 affects the growth and inflammation of RASFs through modulating HBP1. RASFs were transfected with pcDNA, circ_0000396, circ_0000396 + si-NC, or circ_0000396 + si-HBP1. a, b Transfection efficiency was analyzed by using qRT-PCR or western blot. c, d The proliferation of RASFs was determined using colony formation assay and CCK-8 assay. e The apoptosis rate of RASFs was detected using flow cytometry. f The expression of Ki67, cleaved caspase3 and cleaved PARP expression in RASFs was detected using western blot. g–j Levels of inflammatory cytokines IL-6, IL-1β, IL-8 and TNF-α in RASFs was detected by ELISA assay. ^*p < 0.05, ^**p < 0.01, ^***p < 0.001, ^****p < 0.0001 (ANOVA)