Fig. 6

The negative regulation of miR-363-3p on CRC progression could be reversed by CXCL5. a, b The transfection efficiencies of miR-363-3p mimic and pcDNA CXCL5 overexpression plasmid were assessed by detecting miR-363-3p expression and CXCL5 protein expression in SW620 and SW480 cells using qRT-PCR and WB analysis, respectively. c–n SW620 and SW480 cells were transfected with miR-con, miR-363-3p, miR-363-3p + pcDNA or miR-363-3p + CXCL5. c, d The protein expression of CXCL5 was measured by WB analysis. CCK8 assay (e, f), colony formation assay (g), flow cytometry (h–j), and transwell assay (k, l) were used to examine the viability, number of colonies, apoptotic rate, cell cycle, migration and invasion of cells. m, n The protein expression levels of E-cadherin and N-cadherin were detected by WB analysis. *p < 0.05, **p < 0.01, ***p < 0.001