Fig. 7

Hsa_circ_0000231 mediated CRC development by binding to miR-622. HCT116 and SW620 cells were transfected with si-NC, si-hsa_circ_0000231#1, si-hsa_circ_0000231#1 + anti-NC and si-hsa_circ_0000231#1 + anti-miR-622, respectively. A MiR-622 expression was detected by qRT-PCR in HCT116 and SW620 cells. B, C The viability and colony-forming ability of HCT116 and SW620 cells were detected by MTT and colony-forming assays, respectively. D, E Cell cycle and apoptosis were detected by DNA content quantitation assay and Annexin V-FITC and PI double staining assay, respectively, in HCT116 and SW620 cells. F Caspase-3 activity was detected by caspase-3 activity assay in HCT116 and SW620 cells. G, H The migration and invasion of HCT116 and SW620 cells were detected by wound-healing and transwell invasion assays, respectively. I Western blot analysis was performed to reveal the influences between hsa_circ_0000231 silencing and miR-622 downregulation on the protein expression of Bax, t-caspase 3, C-caspase 3, MMP2 and MMP9 in HCT116 and SW620 cells. *p < 0.05 (ANOVA with Tukey’s test)