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Fig. 2 | Journal of Biological Research-Thessaloniki

Fig. 2

From: Nesfatin-1 protects H9c2 cardiomyocytes against cobalt chloride-induced hypoxic injury by modulating the MAPK and Notch1 signaling pathways

Fig. 2

Nesfatin-1 protected H9c2 cells against CoCl2-induced cytotoxicity. A H9c2 cells were treated with nesfatin-1 (10, 20, 40, 60, 80, 100 and 120 nM) for 24 h, and cell viability was detected by MTT assay. BG H9c2 cells were treated with 800 µM CoCl2 for 48 h before treatment with different concentrations of nesfatin-1 (20, 60 and 80 nM) for 24 h. B Cell viability was detected by MTT assay. Cell apoptosis was evaluated using TUNEL staining assay (CE) and flow cytometry (D, F). G The release of LDH in the supernatant was detected using corresponding detection kit. Data are presented as mean ± SD. * <  0.05 vs. control group (0 µM CoCl2); #p  <  0.05 vs. CoCl2 alone group (800 µM CoCl2 and 0 nM nesfatin-1)

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