Fig. 2

Nesfatin-1 protected H9c2 cells against CoCl₂-induced cytotoxicity. A H9c2 cells were treated with nesfatin-1 (10, 20, 40, 60, 80, 100 and 120 nM) for 24 h, and cell viability was detected by MTT assay. B–G H9c2 cells were treated with 800 µM CoCl₂ for 48 h before treatment with different concentrations of nesfatin-1 (20, 60 and 80 nM) for 24 h. B Cell viability was detected by MTT assay. Cell apoptosis was evaluated using TUNEL staining assay (C, E) and flow cytometry (D, F). G The release of LDH in the supernatant was detected using corresponding detection kit. Data are presented as mean ± SD. *p  <  0.05 vs. control group (0 µM CoCl₂); #p  <  0.05 vs. CoCl₂ alone group (800 µM CoCl₂ and 0 nM nesfatin-1)