Fig. 1

Schematic illustration of the cloning procedure, starting with RT-PCR from placenta tissue, derived from a healthy individual, followed by TA cloning of the a-globin CDS and then TA cloning of amplified TAT-α-globin-HA and α-globin-HA into pCRII-TOPO vector. Then, «sticky ends» cloning was conducted, with the restriction enzymes NdeI and XhoI, to generate the recombinant expression vectors pET-16b-TAT-α-globin-HA and pET-16b-α-globin-HA