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Fig. 5 | Journal of Biological Research-Thessaloniki

Fig. 5

From: PTD-mediated delivery of α-globin chain into Κ-562 erythroleukemia cells and α-thalassemic (HBH) patients’ RBCs ex vivo in the frame of Protein Replacement Therapy

Fig. 5

Size exclusion HPLC chromatograms: A hemoglobin tetramer (64.5 kDa) (tR = 21.1 min); B soluble (in PBS 1 ×) sample protein (∼36 kDa) (tR = 23.628 min); C soluble monomer 10xHis-XaSITE-TAT-β-globin-HA (∼20.6 kDa), prior incubated with DTT for 48 h (tR = 25.6 min); D soluble monomer 10xHis-XaSITE-α-globin-HA (∼18.7 kDa), prior incubated with DTT for 48 h (tR = 25.6 min)

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Journal of Biological Research-Thessaloniki

ISSN: 2241-5793

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