HaloDom: a new database of halophiles across all life domains
© The Author(s) 2018
Received: 20 October 2017
Accepted: 20 December 2017
Published: 15 January 2018
Halophilic organisms may thrive in or tolerate high salt concentrations. They have been studied for decades and a considerable number of papers reporting new halophilic species are being published every year. However, an extensive collection of these salt-loving organisms does not exist nowadays. Halophilic life forms have representatives from all three life domains, Archaea, Bacteria and Eukarya. The purpose of this study was to search for all documented halophilic species in the scientific literature and accommodate this information in the form of an online database.
We recorded more than 1000 halophilic species from the scientific literature. From these, 21.9% belong to Archaea, 50.1% to Bacteria and 27.9% to Eukaryotes. Our records contain basic information such as the salinity that a particular organism was found, its taxonomy and genomic information via NCBI and other links. The online database named “HaloDom” can be accessed at http://www.halodom.bio.auth.gr.
Over the last few years, data on halophiles are growing fast. Compared to previous efforts, this new halophiles database expands its coverage to all life domains and offers a valuable reference system for studies in biotechnology, early life evolution and comparative genomics.
Halophiles are extremophile or extremotolerant organisms that can survive in high salinity. They are categorized as slight, moderate and extreme, depending on their maximum salinity tolerance . Halophilic species exist across all life domains [2, 3] showing considerable diversity in metabolic strategies and physiological responses, especially among microbes [1, 4–6]. Research on halophiles has mainly focused on the specific adaptations and molecular mechanisms that enable them to maintain their osmotic balance under salt-stress [7–9]. A great deal of interest has also been channeled towards the investigation of their diversity and phylogenetic relationships as the highest majority of them constitute ancient evolutionary lineages [10, 11]. On a different avenue, biotechnology has recently decided to delve deep into the survival kits of extremophiles in the hunt of biocatalysts functioning in hostile environments. All this interest is reflected in the plethora of papers reporting new halophilic species every year [12–14], a trend which is expected to increase. As a consequence and due to the large quantities of data produced by next-generation sequencing, there is a need for a database repository of extremophiles which will be regularly updated.
So far, there are three halophilic databases available online: HaloWeb , HaloBase  and HProtDB . HaloWeb focuses on genome information and provides complete genome sequences available for downloading. There are also features like blasting sequences against a genome and genomic maps. There are 10 haloarchaeal species registered in total. HaloBase contains more general information in 23 halophilic archaeal and bacterial halophiles. GenBank sequence numbers, number of chromosomes and plasmids, gene/protein content and cellular features are among the database entries. HaloBase provides user accounts, followed by the ability to add a new organism as a registered member. In HProtDB, the first priority is protein content. The resource contains physical and biochemical properties of halophilic proteins for 21 strains of Archaea and Bacteria. It also allows users to register as members and enter their own halophilic data. All three databases are restricted to information about halophilic Archaea and Bacteria, their number of entries is limited to an average of 18, and are irregularly updated.
In this work, we report on a new halophiles database covering more than 1000 halophilic species and spanning all life domains. This new resource named “HaloDom” can be accessed at http://halodom.bio.auth.gr.
An extensive literature search has been carried out through the Web of Science, Scopus, PubMed and Google Scholar using appropriate keywords (i.e. haloph*, salt, saline, hypersaline, extremophile) as well as combinations of them. Ultimately, the Web of Science was chosen as the primary source of literature as it provided a sophisticated search/query engine that suited our methodology and was proven to contain most of the papers found in other literature databases. The keyword combination that returned most papers in Web of Science was “sp nov haloph*” (on title section), returning 610 papers reporting new halophilic species up to 2017. The same keyword combination returned many results in Google Scholar (2410), but not all of these papers contained the desirable keywords in their titles making its search engine unsuitable for our purposes. Scopus returned 615 results, but the interface of Web of Science offered a more flexible environment. There was great overlap among all three databases. Google Scholar however also returned a lot of unrelated papers. Finally, a small number of books and reports containing useful information about halophilic species (albeit with no salinity data) were also included.
Taxonomy databases that were used to record taxonomic information about halophiles in HaloDom
Number of species
NCBI taxonomy browser
World Register of Marine Species
Encyclopedia of Life
Integraded Taxonomic Information System
Atlas of Living Australia
Catalogue of Life
Global Biodiversity Information Facility
INPN—Inventaire National du Patrimoine Naturel
Marine species identification portal
After importing the spreadsheet to the database all data were converted from a csv file to a table called “halodb”. The table was assigned with a primary key column called “Species_ID”. A primary key in mySQL is a number for each individual row of a table and it is unique. In this case, every halophilic species has a unique primary key. This primary key, or “Species_ID” column, always contains an integer starting from 1 and set to “auto-increment”. As more species are added to the database, this number is automatically increased providing every species with its distinctive ID number.
HaloDom’s data structure started as one table that contained all information. However, as data volume increases it is necessary to break down the database into several tables. This methodology improves the speed and efficiency of the database during user query. It is also a way of organizing data, so that administrators can easily check the data integrity, make changes and reduce redundancy. The structure of the database was changed from the table called “halodb”, containing all recorded information, to three tables. The first information separated from “halodb” was the “Bibliography” column, which moved to a table called “Bibliography”. “Bibliography” table was assigned a primary key called “Biblio_id” and four columns: “pub_title” which contains the title of the study, “authors” containing the study’s author/s, “journal” mentioning the name of the journal and “biblio_link” providing a direct link to the study.
“Contact” section lists the administrators and contact information. “About” page shows the date of creation of HaloDom, current number of registered halophilic species and the database version.
We present HaloDom, a database hosting information on more than 1000 halophilic species. This new resource expands considerably compared with previous databases in terms of coverage (representatives from all life domains) and number of entries. Periodical updates are scheduled once every 2 months and as the database grows additional metadata (e.g. geographic distribution, biochemical properties etc.) and analytical tools are planned to be incorporated. For database expansion, we envisage summoning an international panel of experts on extremophiles and engaging the international community from various fields.
Occasionally, during data curation and annotation, species nomenclature proved to be a challenge. This was especially true for Archaea and Bacteria given their notoriously difficult taxonomy and the fast discovery of new strains . We invested considerable efforts into resolving this issue by using several taxonomy databases (see Table 1) but we also encourage user feedback. A grey picture also exists in the literature regarding threshold values in halophile classification (slight/moderate/extreme). For example, in one study the copepod Cletocamptus retrogressus was found in 2–7.4% (w/v) salinity, and thus categorized as slight to moderate halophile, while in another study the recorded salinity range was 19.8–36% (w/v), characteristic of extreme halophiles. This probably reflects the limited knowledge on the biology of many species but as additional data are gathered more accurate annotations are expected. Also, in the light of idiosyncratic molecular mechanisms and signatures in extreme halophilic Archaea [7, 23], criteria for halophile classification could be refined.
The current database can be used as a useful repository and starting point for a wide range of research topics. Over the last few years, investigations have focused on the mechanisms responsible for modulating survival in hypersaline settings [3, 24–28], on the biotechnological production of halophile macromolecules [29, 30], on the phylogenetic position of halophiles in the tree of life , on climate change [32, 33] and even on astrobiology . It is therefore obvious that halophile research addresses appealing questions to several fields of biology, especially in combination with the diverse spectrum of extremophile organisms. As pointed out by de Lorenzo , extremophiles reframe the window of viability. The answer to the basic question whether sustaining life in physicochemical extremes is a matter of entire adaptation or due to the action of a few genes is crucial, multidisciplinary and influential.
AL and IK performed all searches, designed the database, analyzed and interpreted the data. AL, IK and TJA drafted the manuscript. TJA conceived the idea of creating this database and corrected the final version of the manuscript. All authors read and approved the final manuscript.
The current work is part of the Ph.D. of AL and it has been partially supported by the Research Committee of Aristotle University of Thessaloniki (Project No: 94276).
The authors declare that they have no competing interests.
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The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.
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